Draft genome sequence of Staphylococcus auricularis PAPLE_T1 isolate of Carica papaya revealing biosynthetic clusters for beneficial metabolites.

Genomic features of Staphylococcus auricularis PAPLE_T1 isolated from waste sample of Carica papaya obtained from Lagos State, Nigeria, revealed its putative capability to synthesize valuable secondary metabolites. S. auricularis PAPLE_T1 has a 2.4 Mb genome and could be useful as biological agro-antibiotics, for soil bioremediation and in biotechnological industry.

Alkaline-extracted cyanide from cassava wastewater and its sole induction of chromosomal aberrations in Allium cepa L. root tips.

Cassava, a staple crop in Nigeria, processed by numerous factories in rural and suburban locations is known to contain some level of cyano compounds. Lack of stringent environmental regulations on the management of cassava wastewater (CWW) from cassava-processing factories had led to its indiscriminate discharge on the environment. CWW samples were obtained from cassava-processing factories from selected states (Lagos (A), Oyo (B), Ogun 1 (C1), Ogun 2 (C2) and Cross River (D)) in Nigeria to determine the cytotoxic and genotoxic effects of extracted cyanide from the wastewaters. The cyanide was hydrolysed via chemical degradation utilizing 1.25 M NaOH and subsequently titrated using silver nitrate with p-dimethylaminobenzalrhodamine as indicator. Further, in order to explore the potential toxicity of this pollutant present in the effluent, a battery of short-term biological assay (Allium cepa chromosomal aberration test) was used. Bulbs with roots of Allium cepa L. were treated with different concentrations (0.05%, 0.1%, 0.2%, 0.4% and 0.8%) of CWW, and after 48 h, the root tips were processed for cytological studies by the aceto-orcein squash procedure. The results revealed that cyanide concentrations on re-fluxing were in the range of 1.0 and 1.3 mg/L. All concentrations induced a number of chromosomal aberrations in the root tip cells. The mitotic index decreased significantly (p < 0.05) with increasing concentration. The cytotoxic effects showed strong concentration-dependent root growth inhibition with EC50 values of 30%, 20%, 37%, 43% and 22% for A, B, C1, C2 and D, after 72 h. The findings thus indicate that alkali treatment is very efficient in degrading the cyanide content of CWW and has shown that the combination of physico-chemical analysis along with the sole toxicity assessment could provide valuable information about the sole toxicity of cyanide as a chemical pollutant present in the cassava effluent.

Ecological impact of organochlorine pesticides consortium on autochthonous microbial community in agricultural soil.

Organochlorine pesticides (OCPs) used in agricultural practices are of global concern due to their toxicological hazards on biomes of the impacted soil. Geochemistry and microbiome of OCPs-impacted (OW) soil was determined and compared with those of pristine (L1) soils. Microbiome of OW was based on sequencing total 16S rRNA genes of prokaryotes and Internal Transcribed Spacer (ITS2) regions between 5.8S and 28S rRNA genes of eukaryotes using Illumina MiSeq platform for bacterial and fungal communities, respectively. Geochemical properties of OW were assessed for ecological risks of OCPs on biota via risk quotient (RQ) and maximum cumulative ratio (MCR). It was established OW was polluted with 15 OCPs, along with consequential nitrate and phosphorous deficiencies. Ten of the 15 OCPs exerted severe ecological risk (RQ > 1: 4-992), of which endosulfan contributed 76% of the ecotoxicity (MCR = 1.3) on OW. The key players in OW were observed to be Enterobacteriaceae and Mortierellaceae represented by Escherichia and Mortierella taxa, respectively. Low abundance of Nitrospirae species and extinction of Glomeromycota in OW connoted serious toxicological consequences of the OCPs. Taxon XOR (Taxon Exclusive Or) analysis revealed 38,212 and 63,474 counts of bacterial and fungal species, respectively, were exclusively found in the impacted OW and possibly contributed to natural attenuation of the OCPs in the impacted agricultural soil. Conversely, 61,005 (bacteria) and 33,397 (fungi) species counts that were missing in OCPs-impacted OW, but present in pristine L1, opined the species as bio-indicators of OCPs ecotoxicity in agricultural soils. While the species tagged as bio-indicators would be valuable in monitoring OCPs pollution, those suggested to be players in self-recovery process will be invaluable to designing bioremediation strategies for OCPs-impacted agricultural soil.

Metal biouptake by actively growing cells of metal-tolerant bacterial strains.

Metal uptake potentials of Pseudomonas aeruginosa CA207Ni, Burkholderia cepacia CA96Co, Rhodococcus sp. AL03Ni, and Corynebacterium kutscheri FL108Hg were studied to determine their competence in detoxification of toxic metals during growth. Metabolism-dependent metal biouptake of the bacteria revealed appreciable uptake of the metals (57-61, 10-30, 23-60, and 10-16 mg g dw(-1) of Ni(2+), Cr(6+), Co(2+), and Cd(2+), respectively) from medium, after initial drop in pH, without lag phase. The bacteria exhibited 95-100% removal efficiency for the metals from aqueous medium as 21 (±0.8)-84 (±2.0) concentration factors of the metals were transported into the bacterial systems. Passive adsorption onto the cell surfaces occurred within 2-h contact, and afterwards, there was continuous accumulation for 12 days. Biosorption data of the bacteria were only fitted into Langmuir isotherm model when strains AL96Co, CA207Ni, and AL03Ni interacted with Ni(2+), achieving maximum uptake of 9.87, 2.72, and 2.69 mg g dw(-1), respectively. This study established that the actively growing bacterial strains displayed, at least, 97.0% (±1.5) continuous active removals of metals upon adsorption. The bacteria would be good candidates for designing bioreactor useful in the detoxification campaign of heavy metal-polluted systems.

Mercury bioremoval by Yarrowia strains isolated from sediments of mercury-polluted estuarine water.

Difference in mercuric ion removal by resting and growing cells of two mercury-resistant yeast strains, identified as Yarrowia spp. (strains Idd1 and Idd2), were studied. Resting cells of strain Idd2 exhibited high maximum Hg(2+) removal capacity (59 mg mercury per g dry cell weight [gdw(-1)]) by adsorption than those of resting cells of strain Idd1 (32 mg gdw(-1)). The resting cells of strain Idd2 exhibited a higher Hg(2+) desorption capacity using CaCl2 (68 %) and EDTA (48 %) than strain Idd1, depicting weaker binding of Hg(2+) onto strain Idd2 unlike strain Idd1. The actively growing yeast cells showed opposite Hg removal characteristics to those of the resting cells. Strain Idd1 adsorbed less Hg(2+) from culture medium supplemented with Hg(2+) than strain Idd2. However, the growing strain Idd1 reduced and vaporized 27 % of supplemented Hg(2+) as metallic mercury (Hg(0)), while the growing strains Idd2 vaporized 15 % of the supplemented Hg(2+). These two yeast strains are potential biotechnological tools for the eventual bioremediation of polluted aquatic systems.

Biodegradation of petroleum hydrocarbons in the presence of nickel and cobalt.

Bioremediation of environments co-contaminated with hydrocarbons and heavy metals often pose a challenge as heavy metals exert toxicity to existing communities of hydrocarbon degraders. Multi-resistant bacterial strains were studied for ability to degrade hydrocarbons in chemically defined media amended with 5.0 mM Ni(2+), and Co(2+). The bacteria, Pseudomonas aeruginosa CA207Ni, Burkholderia cepacia AL96Co, and Corynebacterium kutscheri FL108Hg, utilized crude oil and anthracene without lag phase at specific growth rate spanning 0.3848-0.8259 per day. The bacterial populations grew in hydrocarbon media amended with nickel (Ni) and cobalt (Co) at 0.8393-1.801 days generation time (period of exponential growth, t = 15 days). The bacteria degraded 96.24-98.97, and 92.94-96.24% of crude oil, and anthracene, respectively, within 30 days without any impedance due to metal toxicity (at 5.0 mM). Rather, there was reduction of Ni and Co concentrations in the axenic culture 30 days post-inoculation to 0.08-0.12 and 0.11-0.15 mM, respectively. The metabolic functions of the bacteria are active in the presence of toxic metals (Ni and Co) while utilizing petroleum hydrocarbons for increase in biomass. These findings are useful to other baseline studies on decommissioning of sites co-contaminated with hydrocarbons and toxic metals.

Chromium (VI) biosorption properties of multiple resistant bacteria isolated from industrial sewerage.

Chromium (VI) [Cr (VI)] biosorption by four resistant autochthonous bacterial strains was investigated to determine their potential for use in sustainable marine water-pollution control. Maximum exchange between Cr (VI) ions and protons on the cells surfaces were at 30-35 °C, pH 2.0 and 350-450 mg/L. The bacterial strains effectively removed 79.0-90.5 % Cr (VI) ions from solution. Furthermore, 85.3-93.0 % of Cr (VI) ions were regenerated from the biomasses, and 83.4-91.7 % of the metal was adsorbed when the biomasses was reused. Langmuir isotherm performed better than Freundlich isotherm, depicting that Cr (VI) affinity was in the sequence Rhodococcus sp. AL03Ni > Burkholderia cepacia AL96Co > Corynebacterium kutscheri FL108Hg > Pseudomonas aeruginosa CA207Ni. Biosorption isotherms confirmed that Rhodococcus sp. AL03Ni was a better biosorbent with a maximum uptake of 107.46 mg of Cr (VI) per g (dry weight) of biomass. The results highlight the high potential of the organisms for bacteria-based detoxification of Cr (VI) via biosorption.